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Medlem
James John C.V.

James John, Ph.D

Postdoctoral researcher

The Life Science Centre (Room: P2315)

School of Science and Technology 

Örebro University, SE 701 82 Örebro, SWEDEN

Email: james.john@oru.se; james_cmc2002@yahoo.co.in

Phone: +91-9841248402, Mobile:-+460728480701

·  Expertise in biological sciences research, molecular biology, microbiological, immunological techniques, and genomics 

·  Experienced in molecular testing, PCR, qPCR, ddPCR, gene sequencing and research related computer applications

·  Detail oriented and multitasking professional with exceptional troubleshooting, technical problem solving, analytical and management skills

·  Have published over 33 research papers in reputed national and international journals

 

 

 

Competencies

  • Animal cell culture and cell-based assay
  • Western Blotting
  • Flow cytometer-based assay
  • Real time PCR, qPCR and ddPCR
  • Microbiology techniques
  • External Quality Assurance Scheme (EQAS)
  • Bioinformatics techniques

Academics

 

2018 - Doctor of Philosophy (Ph.D. in Biotechnology) - School of Life Science, Pondicherry University

2009 - M.Sc. Medical Laboratory Technology - Loyola College, Madras University 76 % Distinction

2008 - P G Diploma in Hospital Management - Madurai Kamaraj University (Correspondence) 66%

2005 - B.Sc. Medical Laboratory Technology - Christian Medical College & Hospital Vellore 72%                       

 

Awarded CSIR-NET 2018 Lectureship

 

Primary Education

Intermediate Science (12th), 2000, Bihar Intermediate Education Council Patna, Bihar, 69 %

Secondary School Leaving Certificate (SSLC), Class 10, 1998, Bihar Secondary School Examination, Patna, Bihar, 74 %

 

 

Professional Experience

 

Department of science and technology, Orebro University, Sweden (March 2020 till Date)

Post Doctorate fellowship

·         Working to develop highly sensitive ddPCR analyses for detecting biological threats and indicator microbial species for detecting biological and chemical pollutants in environmental freshwater and drinking water

·         Writing research proposal, Guiding Ph.D. students in support with PI, conducting own research and compiling

 

Central Research Facility, Sri Ramchandra Medical University, Chennai, India (July 2018 to date)

Adjunct research faculty

Coordinate with investigators to prepare presentations / reports, research publications of studies                    

 

Clinical Microbiology, Christian Medical College & Hospital, Vellore (Aug 2017 to Mar 2018)

Post Doctorate researcher

·         Communicate with research collaborators and present research at group meetings

·         Prepare study-related documentation like protocol, manuals, institutional review board documents

·         Collaborate with investigators to prepare reports of research work and progress reports

 

Department of Biotechnology, Pondicherry University (July 2012 to May 2017)

Senior Research Fellow

Experienced in animal cell culture and cell-based assay, qPCR, and Western Blotting, Flow cytometer

 

Clinical Epidemiology and Microbiology, Christian Medical College & Hospital, Vellore (July 2009 to June 2012)

Research Associate (Lab Co-ordinator)

Project 1. Vaccine preventable - invasive bacterial disease surveillance – Southeast Asia (WHO-SEARO, ICMR), July 2009-June 2012.

Project 2. Neonatal Invasive Bacterial Disease Surveillance (ICMR), 2011-2012.

Work Profile:

  • External Quality Assurance Scheme (EQAS) for sentinel Site
  • Pneumococcal serotyping by PCR and Real time PCR,
  • Practiced in Essential Document Preparation (SOPs)

 

Guru Raghvendra Hospital, K.K Nagar, Chennai (2008)

Tutor

Christian Medical College & Hospital, Vellore-4                    (Oct 2005 to Oct. 2006)

Interns Trainer

 

 

Research Presentation at CMC Vellore Institutional Annual Research Day

·         Detection of Pan-Aminoglycoside-Resistant Gram-Negative Bacteria by Molecular method. 2010

·         Determination of S.pneumoniae serotype by two different method namely conventional antisera based Co-agglutination and Sequential Multiplex PCR. 2010

·         Antimicrobial activity of Fosfomycin against uro-pathogens from community and hospitalized patients at a tertiary care hospital in South India 2010

·         Customized Sequential multiplex PCR assay to determine capsular serotypes of invasive S pneumoniae for India 2011

·         Phenotypic and molecular characterization of Vancomycin-resistant enterococcus (VRE) isolated from tertiary care center in south India. 2011

 

Certificate courses on Clinical Research

Good Clinical Practice - National Institute on Drug Abuse (NIDA) and Center for Clinical Trials (CCTN)

Design and Interpretation of Clinical Trials - online-Johns Hopkins University

Data Management for Clinical Research – online -Vanderbilt University

Epidemics - the Dynamics of Infectious Diseases - online-The Pennsylvania State University

Epidemiology: The Basic Science of Public Health - online-The University of North Carolina

Introduction to Genomic Technologies - online-Johns Hopkins University

 

Other Certifications

Certificate in Computer Application, 1997-98, Khrist Raja High School, Bettiah, West Champaran, Bihar

Epidemiology: The Basic Science of Public Health: an online non-credit course authorized by The University of North Carolina at Chapel Hill and offered through Coursera.

Epidemics - the Dynamics of Infectious Diseases: an online non-credit course authorized by The Pennsylvania State University and offered through Coursera

 

 

Text Box: Research Projects Ph.D thesis Research Investigations on Antimicrobial and Anticancer properties of Quorum sensing signalling molecules produced by Nosocomial pathogen Acinetobacter baumannii M.Sc Dissertation Research Investigation of Rickettsial Diseases In Patients Diagnosed With Fever of Unknown Origin By Conventional And Molecular Methods. (ICMR), 2008-2009. (Six Month) Research Associate Vaccine preventable - invasive bacterial disease surveillance – Southeast Asia (WHO-SEARO, ICMR, CMC Vellore), July 2009-June 2012 Co-Investigator Antimicrobial activity of fosfomycin against uro-pathogens from community and hospitalized patients at a tertiary care hospital in South India. (Fluid Research Grant CMC Vellore), 2010-2011 Professional Training Molecular methods for disease diagnosis Centre of Disease and control and prevention (CDC), Atlanta and World Health Organization (WHO), Geneva 2011 Real time PCR Training Life Technologies at the state of art training facility at Gurgaon, India 2011 Animal cell culture Hands on training by National Centre for Cell Science, Pune (NCCS) at the training facility of DBT-IPLS lab Pondicherry University, India 2013 National Eligibility Test for Assistant Professor University Grants Commission 2018 Fellowship and Awards • Post-doctoral research fellowship, Örebro University, SWEDEN • Senior Research Fellowship - Department of Biotechnology, New Delhi, India • DBT Interdisciplinary Program in Life Sciences at the Department of Biotechnology, Pondicherry Central University, India (2012-2016) • Travel grant Awarded by Indian Association of Medical microbiologists to attend 40th Annual Conference of Indian Association of Medical Microbiologists (2016) • Travel grant Awarded by Bill and Melinda Gates to attend “International symposium on pneumococci and pneumococcal disease (ISPPD 2014) Research Publications

Total Publications: 32               

Total citations: 201

i10 Index: 13  

h-index: 12

Full details at

https://scholar.google.co.in/citations?user=nRq-hSwAAAAJ&hl=en

and at https://www.linkedin.com/in/dr-james-john-ph-d-09219333/

Key publications                                                                                 

- James John, Rajagopalan Saranathan, Lakshmi Narayana, Vasanth Thamodharan, Satya Prakash Singh, T. Pragna, Adigopula Lakshmi, Mallu Abhiram CharanTej, R. Srinivasa Rao, R. Krishna, Surya Prakash Rao H , K. Prashanth. The quorum sensing molecule N-Acyl homoserine lactone produced by Acinetobacter baumannii displays antibacterial and anticancer properties. Biofouling. 2016; 32 (9): 1029–1047 (Impact factor 4.0)

- James John, Kripa Shanker Kasudhan, Reba Kanungo, Savitri Sharma, Vaishali Dohe. Distribution of different genes responsible for invasive characteristics, detection of point mutations in capsular gene wchA and biofilm production among the invasive and noninvasive isolates of Streptococcus pneumonia. Indian Journal of Medical Microbiology. Accepted. 2017 (Impact factor 1.1)

- James John. The Remaining Challenges to Laboratory-based Surveillance of Invasive of pneumococcal diseases. Indian Pediatrics. 2015; 52: 199-200. (Impact factor 1.1)

- Noura H. Abd Ellah, Hesham M. Tawfeek James John and Helal F. Hetta. Nanomedicine as a Future Therapeutic approach for Hepatitis C virus (Accepted 2019) Nanomedicine Impact factor 5.2

- Rehab Mahmoud Abd El-Baky, Helal F Hetta, Gamal E. Abuo-Rahma, Tim Sandle, James John. A novel mechanism of action of ketoconazole: inhibition of NorA efflux pump system and biofilm formation in Staphylococcus aureus. Infection and drug resistant. (Accepted) (Impact factor 3.5)

Date of Birth: 04/03/1983    

Gender: Male

Nationality: Indian

Language Proficiency: Hindi, English, Tamil (Basic)

 

Address

No. 2, Thirupurkumaran. 4th street, Puzhal, Chennai

Tamilnadu 600066, India



2025-05-13 15:43
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